Gut Mycobiome Maturation and Its Determinants during Infancy: A Comparison of Shotgun Metagenomic and ITS2 Amplicon-Based Sequencing Approaches

Samples from subjects recruited as part of the "Microbiome, Antibiotic and Growth Infant Cohort" (MAGIC) study. Fecal samples from enrolled children born at Pennsylvania Hospitals were collected within a few days of birth and then every three months until the infant reached 24 months of age. Demographic information, including antibiotic usage and exposure, was collected at every time point for each subject. DNA was extracted and sequenced using two methods: shotgun metagenomics and ITS2 amplicon sequencing. This dataset can be used to compare and contrast sequencing methods in the context of the developing infant gut mycobiome in both antibiotics-exposed and control infant populations.