DNA was extracted within 24 hour using the MoBio PowerSoil DNA Isolation kit (Qiagen, Valencia, CA, USA). Nested PCR was used to amplify the fungal internal transcribed spacer 1 (ITS1) using barcoded-
Anaerobic fungi (Neocallimastigomycota) are common inhabitants of the digestive tract of large mammalian herbivores, where they make an important contribution to plant biomass degradation. The interna
The ITS1 sequences were aligned in the online version of MAFFT 7 (Katoh, Rozewicki, & Yamada, 2017) under the Q-INS-i algorithm with the default settings. Gaps were coded as characters following t
