Epigenetic profiling of microglia-specific gene transcription and chromatin accessibility in mouse forebrain after chronic mild stress

Multiple studies have suggested that stress induces neuroinflammation and contributes to emotion-related behavioral deficits. To study epigenetic changes in microglia after chronic stress, we purified microglia from mouse brain after exposure to chronic unexpected mild stress (CUMS), and then performed RNA-seq and ATAC-seq to study microglia-specific alterations of transcriptome and chromatin accessibility in response to chronic stress. Microglia-specific ATAC-seq and RNA-seq in stress and control mice, 3 animals per group.