Analysis of the replication and early transcription of Pseudorabies virus by Long Read Nanopore Sequencing in different time points of infection

PK-15 cells were infected by PRV with 10 MOI in three replicates. Furthermore PAA treated samples were infected as well in the same manner in three replicates. Thereafter RAN was isolated in 1h, 2h, 4h, 6h, 8h, 12h time points. Oxford Nanopore MinION device according the library preparation protocol of direct cDNA Sequencing Kit (SQK-DCS109).