lung microbiota and hypercapnic AECOPD

The aim of this study is to assess the link between lung microbiota composition and death in critically ill admitted for hypercapnic acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Every patient receiving non-invasive or invasive ventilation for AECOPD admitted to intensive care unit from October 2018 to May 2019 were included (ADMIRE study, NCT03641235). Induced sputum or tracheal aspirate were sampled and DNA extraction was performed by QIAamp® PowerFecal® Pro DNA kit (QIAgen®). V3-V4 regions of 16SRNA coding gene was amplified to analyse the bacterial kingdom of lung microbiota (bacteriobiota) and ITS2 to analyse the fungal kingdom (mycobiota). Sequencing (2x250bp paired-end reads) was performed on MiSeq sequencer (Illumina®) and DADA2 pipeline on R software was used for bioinformatics analyses.