Physiological and Molecular Characterization of an Boron-Resistant Saccharomyces cerevisiae Strain Obtained by Evolutionary Engineering

One of the essential or beneficial micronutrient for plants and animals is boron that is an ultra-trace element. Although boron can inhibit the growth of Saccharomyces cerevisiae around 80 mM, it is also a growth supplement. However, little information is currently available regarding the molecular mechanisms and essentiality of boron. In this paper, the approach was to generate S. cerevisiae mutants with high boron resistance by using evolutionary engineering strategy that was previously applied successfully. Boron-resistant S. cerevisiae mutants were obtained and their phenotypic and physiological characteristics were determined. In order to identify the molecular mechanisms implicated in boron resistance, the whole transcriptomes and genome sequence analysis of wild type and one of the most resistant mutants were compared.硼作为一种超痕量元素，是动植物必需且有益的微量营养素之一。尽管浓度约80 mM的硼会抑制酿酒酵母（Saccharomyces cerevisiae）的生长，但它同时也是一种生长补充剂。然而，目前关于硼的分子作用机制及其必需性的相关研究信息仍较为匮乏。本研究采用此前已成功应用的进化工程策略，构建具有高硼抗性的酿酒酵母突变体。研究成功获得了耐硼酿酒酵母突变体，并对其表型及生理特性进行了测定。为解析硼抗性相关的分子机制，本研究对野生型菌株与一株高抗性突变体的全转录组及基因组序列进行了对比分析。