GSE221058_Gene_exprs_KCKT.xlsx

Total RNAs were extracted from control or 26 mM KCl treated AC16 cells at 16 hrs by RNeasy Kits (Qiagen). Sample quality control, transcriptome library preparation, library quality control, sequencing, raw data output, data quality control and bioinformatics analysis were performed by BGI Americas Corporation (San Jose, CA) and validated by R-programming. Raw reads were filtered to remove the reads containing the adaptor sequence, unknown nucleotides greater than 5%, or a quality score less than 15 by SOAPnuke software . The clean reads were aligned to a reference genome GRCh38.p12 using HISAT or to a reference transcriptome using Bowtie 2 . RSEM was used to calculate the gene expression level of each sample . For detecting differential genes, the DESeq2 method was employed with Qvalue (Adjusted Pvalue) < 0.05 (20) .