Gene expression in Blumeria graminis f. sp. hordeii (Bgh) isolate DH14 during plant pathogenesis

We performed RNA-sequencing of Bgh-infected barley leaves at two different time-points after infection to examine gene expression in the barley powdery mildew isolate DH14 during plant pathogenesis. Overall design: Detached leaves of the barley cultivar Pallas were infected with Bgh isolate DH14. Epidermal peels were used to enrich fungal RNA. The samples were collected at 16 and 48 hours post inoculation (hpi).