Rolling circle DNA replication in vitro by a complex of herpes simplex virus type 1-encoded enzymes.
收藏PubMed Central1994-10-25 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC45082/
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资源简介：
Extracts of insect cells infected with baculoviruses recombinant for the herpes simplex virus 1 (HSV-1)-encoded enzymes that are required for its replication can promote the rolling circle replication of circular plasmid templates. Replication is independent of a HSV-1 origin of replication (oris) or the HSV-1 origin binding protein and is inhibited by the origin binding protein when the plasmid contains oris. Replication is dependent on a complex composed of the HSV-1-encoded DNA polymerase and its processivity enhancing factor (the UL42 protein), ICP8 (the HSV-1-encoded single-strand DNA binding protein), and the HSV-1-encoded helicase-primase. The complex can be purified by size-exclusion and anion-exchange chromatography. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1994-10-25
