Sequences of oligonucleotides used in this study.
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aAll the primers and probes were designed using PRIMER EXPRESS software version 3.0 (Applied Biosystems Inc.).bFAM was used as a 5'-reporter dye and TAMRA as a 3'-quencher dye to design each TaqMan probe.cUnderlines below the nucleotide sequences indicate restriction enzyme cleavage sites. F, forward primer; P, probe; R, reverse primer; O, outer primer; I, inner primer.Sequences of oligonucleotides used in this study.
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2015-12-03
