The second polar body determines the fate asymmetry in the mouse embryo

The second polar body (PB2) of mouse embryo may contains some crucial factors that control fate asymmetry. This study is aimed at discovering differentially expressed genes in PB2 compared with zygote. PB2 and Zygote of 1-cell mouse embryos were separated after removing zona pellucida. Then mRNA profile of collected PB2 and zygote were analysed.小鼠胚胎的第二极体（second polar body, PB2）或携带有调控细胞命运不对称性的关键因子。本研究旨在探究相较于合子（zygote），PB2中存在的差异表达基因。研究人员先去除1细胞期小鼠胚胎的透明带（zona pellucida），分离得到PB2与合子，随后对收集到的PB2及合子的mRNA表达谱（mRNA profile）展开分析。