This study contains raw HiFi FASTQ reads and four haplotype-resolved HiFi genome assemblies for two Pakistani water buffalo breeds, Azikheli and Nili Ravi.

This is a collaborative study between two institutes: the National Institute for Biotechnology and Genetic Engineering(NIBGE) ,Faisalabad, Pakistan, and the Roslin Institute, University of Edinburgh. The study includes four haplotype resolved HiFi genome assemblies of two Pakistani water buffalo (Bubalus bubalis) breeds, Nili Ravi and Azikheli. The animals were sequenced at depths of approximately 34x and 35x using PacBio Revio SMRTbell library preparation, respectively. doi: https://doi.org/10.1101/2025.05.04.652079 (A comprehensive water buffalo pangenome reveals extensive structural variation linked to population specific signatures of selection)