Biogenesis of the Photosynthetic Apparatus in Chromochloris zofingiensis

RNA-Seq of Chromochloris zofingiensis following the transition from heterotrophic growth on glucose to photoautotrophic growth. Overall design: Cultures of Chromochloris zofingiensis were grown diurnally (16 h light, 8 h dark) in 100 µmol photons m^-2 s^-1 medium light (ML) for 72 h in media supplemented with 20 mM glucose (glc+). At t = 0, cultures were transferred to glucose-free media (glc-). Samples were collected in quadruplicate at 0, 0.5, 1, 3, 6, and 12 h for glc+ cultures and at 0.5, 1, 3, 6, and 12 h for glc- cultures. All samples were collected during the 16hr daylight period.