Identification and tracking of alloreactive T cell clones in Rhesus Macaques (RM) through the RM-scTCR-Seq platform

T cell receptor clonotype tracking is a powerful tool for interrogating T cell mediated immune processes We apply this method to a Rhesus Macaque model of Graft Versus Host Disease (GvHD), and identify and track in vitro detected alloreactive clonotypes in GvHD target organs and explore their GvHD driven cytotoxic T cell signature. Overall design: Examination of scTCR-Seq and scRNA-Seq of T cells in RM model of GvHD and in vitro MLR experiments.