Enterococcus faecalis JM263 Alleviates Liver Metabolomics Perturbations in Yaks Fed a High-concentrate Diet

This study employs untargeted metabolomics (LC-MS/MS) to investigate the metabolic reprogramming in the liver of yaks in response to Enterococcus faecalis JM263 supplementation under a high-concentrate diet (HCD). Yaks were divided into a control group (HCD) and a treatment group (HCD + E. faecalis JM263). Following the feeding trial, liver tissues were collected and quenched immediately in liquid nitrogen. Metabolites were extracted and analyzed using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). The study aims to identify differential metabolites and enriched metabolic pathways