Unstranded RNA-seq profiling of cerebral cortices from Taf8-deleted and Taf8-intact mouse embryos

Taf8 was deleted in the mouse central nervous system using our Taf8 conditional allele. To determine transcriptional changes due to Ta8 deletion in the developing CNS, unstranded RNA sequencing was performed on cortices from Taf8 deleted embryos (E14.5 Taf8lox/–NesCreT/+p53–/–) and control embryos (E14.5 Taf8+/+NesCreT/+p53–/–). Overall design: Four Taf8-deleted and four control samples were prepared, each from a separate mouse embryo. Unstranded RNA sequencing was undertaken on an Illumina HiSeq 2000 to produce 100bp paired-end reads.