Studies on a component of the plant nuclear lamina

Guanylate Binding Proteins (GBPs) play key roles in interferon-dependent immunity in mammals, but have largely unknown functions in other eukaryotes. Here, we aim to functionally characterize GBPb (At5g46070) - one of the three GBP-orthologues of the model plant Arabidopsis thaliana. To start elucidating the role of GBPb in plants, we analyse and compare transcriptomes of an Arabidopsis knockdown line with significantly reduced expression of GBPb, the complemented line expressing GBPb-GFP in the background of the knockdown, a mutant (mail1) with root growth defects resembling those of the gbpb mutant, and corresponding WT seedlings as control.