Chromomal translocations induced by CRISPR-Cas9 persist in expanded T cells

Chromosomal translocations cause various lymphoid cancers and are prevalent in CRISPR-Cas9-meidated gene editing. However, the fate of gene editing-induced translocations remains elusive in vivo. Here, we found that translocations in engineered T cells experience clonal expansion after transferring into recipients for three weeks and remains comparable level with edited T cell before transfer, arguing against the notion that translocations would be gradually lost during cell proliferation due to growth retardation. Examination of the fate of chromosomal translocation induced by gene editing in T cells