The comprehensive analysis of total expression profiles in initial diagnosed multiple myeloma by genetic microarray

Long non-coding RNA (lncRNA) plays a vital role in Multiple Myeloma. Nevertheless, the exact expression features and functions of lncRNAs are still obscure.To investigate aberrantly expressed lncRNAs and mRNAs in MM, we screened the crucial differentially expressed lncRNA and mRNA in mononuclear cells from bone marrow of multiple myeloma patients and normal donors.These differentially expressed lncrnas are likely to play a key role in the development of MM, and may be used as a novel biomarker for the diagnosis or therapy of MM. We used microarrays to screen the candidate differentially expressed lncRNAs and mRNAs in mononuclear cells from bone marrow of multiple myeloma patients and normal donors .Subsequently,the candidate differentially expressed lncRNAs and mRNAs were verified by qRT-PCR. We selected BM mononuclear cells from 10 multiple myeloma patients and 10 normal donors for total RNA extraction and Clariom D Assay (human,Affymetrix) analysis. At the same time, in order to deeply understand the relationship between differential lncRNA and mRNA, we carried out in-depth analysis, including GO Enrichment Analysis,Hierarchical Clustering,Enrichment Analysis,co-expression network and ceRNA network analysis under the basic analysis. We are trying to find out an novel marker which is of guiding significance for the diagnosis and therapy of Multiple Myeloma.