Plant community composition and traits modulate the impacts of drought intensity on soil microbial community composition and function

In an outdoor grassland mesocosm experiment, we determined the interactive effects of increasing drought intensity and grassland plant community resource acquisition strategy on soil bacterial and fungal communities during and after an experimental drought.DNA was extracted from 0.25 g fresh (frozen) soil using the DNeasy PowerSoil Pro Kit (Qiagen, Germany). The 16S rRNA (prokaryotes) and ITS (fungi) marker genes were sequenced on an Illumina MiSeq platform. Prokaryote community composition was determined by sequencing the V4-V5 region of the 16S rRNA gene with primer pair 515F and 926R (Walters et al., 2016). This dataset includes the 16S rRNA data. The ITS (fungal) data can be found at BioProject accession number XXXX.