Epigenome analysis of apoptotic and control cancer cell lines

Genome wide DNA methylation profiling of four control and cancer cell lines where apoptosis was induced using BH3 mimetics. The Illumina Human MethylationEPIC methylation profiling array was used to obtain DNA methylation profiles across approximately 850,000 CpGs. 8 samples were from treated cells harvested at two different time points representing early and late apoptotic stages, and 4 samples were controls combined from the two timepoints. 4 additional samples was left over DNA from the Nalm6 cell line ran on the array to fill up the bead chips. Data analysis will be presented in the manuscript "HPV-induced host epigenetic reprogramming is lost upon progression to high-grade cervical intraepithelial neoplasia" accepted for publication in the International Journal for Cancer. Bisulphite converted DNA from the 16 samples were hybridised to the Illumina Human MethylationEPIC methylation profiling array.