Quantification of substoichiometric tRNA and tsRNA modification sites in human neuronal cell lines upon angiogenin exposure. tsRNA epitranscriptomics

Here, we investigate the stoichiometry of incompletely modified sites in tRNAs and tsRNA from human cells lines (MZ-294 and SH-SY5Y) for their information content. Using a panel of RNA modification mapping methods, we assess the stoichiometry of sites that contain the modifications 5-methylcytidine (m5C) by Bisulfite sequencing, 2’-O-ribose methylation (Nm) by RiboMethSeq and 3-methylcytidine (m3C), 7-methylguanosine (m7G) and Dihydrouridine (D) by AlkAniline Seq. The different cell lines have also been exposed to angiogenin to assess its impact on modification profiles.