Single nuclei RNAseq of human Rett syndrome neurons cortical samples after sorting for MECP2 expression

Here we employed sc-RNA-seq on nuclei from MECP2 Syndrome brain and normal brain to understand the effect of loss of MECP2 on neurons compared to both MECP2+ neurons in isogenic MECP2 Syndrome brain, as well as to WT brain to determine which changes are due directly to loss of MECP2, and in transgenic rats lacking MECP2. From 5 individual Rett syndrome patient brain samples, we isolated nuclei using sucrose gradient centrifugation and sorted using FACS for neurons with low and high MECP2 expression to study transcriptional changes associated with MECP2 mutation.