Effect of depletion of OGT on gene expression during M2 polarization of macrophages. Effect of depletion of OGT on gene expression during M2 polarization of macrophages

To investigate the function OGT in the regulation of M2 polarization of macrophages, we established IL-4-activated bone marrow-derived macrophages (BMDMs) from mice of wild-type control or Lyz2-Cre/Loxp-mediated knockout of OGT. We then performed gene expression profiling analysis using data obtained from RNA-seq of wild-type (WT) and OGT-knockout (OGT-KO) macrophages at two time points during IL-4 (20ng/μl) sitmulation. Overall design: Comparative gene expression profiling analysis of RNA-seq data for WT and OGT-KO BMDMs.