Small RNA sequencing of BYDV-infected barley plants

To examine if the elevation of antiviral defense displayed in transgenic barley plants that overexpressing SnRK1-YFP might be associated with the increased abundance of BYDV vsiRNAs, small RNA sequencing assays were conducted to analyze the vsiRNA accumulation levels in BYDV-infected transgenic barley plants and WT controls. The small RNAs were separated from the total RNAs that extracted from the BYDV-infected barley plants at 4, 7 and 14 dpi, and then were employed for constructing the small RNA-Seq library.