Transcriptome analysis of lung neutrophils from mice infected with Mycobacterium tuberculosis

Neutrophils are accumulated in mouse lungs following Mycobacterium tuberculosis infections. Increased influx of neutrophils is associated with increased susceptibility to disease, but the underlying mechanisms of neutrophil mediated Tuberculosis disease pathogenesis is poorly understood. To understand the transcriptomic differences between resistant (C57 BL/6) and susceptible (C3HeB/FeJ) mouse lung neutrophils, we sorted them at Day 29 post infection and analyzed them by bulk mRNA sequencing. Overall design: Bulk mRNA sequencing to study transcriptomic differences in neutrophils at Day 29 post infection with Mycobacterium tuberculosis HN878 from C57 BL/6 and C3HeB/FeJ mouse lungs.