Genome-wide methylation profiling data for diploid crabapple HCT-DP and its autotetraploid HCT-TP leaves at 40 days after in vitro culture
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The raw sequencing reads (FASTQ files) from this study are unfortunately no longer available due to an unforeseen archive error. As an alternative, we deposite the processed single-base methylation data supporting the findings of this study in the ZenodoThis dataset contains genome-wide, single-base resolution DNA methylation calls for diploid crabapple (Malus spp.) cultivar 'HCT-DP' and its corresponding autotetraploid line 'HCT-TP'. Seedlings of both genotypes were harvested 40 days after in vitro culture, with three biological replicates per genotype (HCT-DP: replicates 1–3; HCT-TP: replicates 1–3), totaling six samples. For each replicate sample, all methylation call files are provided as a compressed archive (.zip) containing three tab-delimited text (.txt) files classified by sequence context: *_CG.txt: methylation at CG sites *_CHG.txt: methylation at CHG sites (H = A, C, or T) *_CHH.txt: methylation at CHH sites (H = A, C, or T) Each .txt file includes the following columns: unique site identifier (chrBase), chromosome name (chr), genomic coordinate (base, 1-based coordinates), strand (strand), read coverage depth (coverage), percentage of methylated cytosines (freqC), and percentage of unmethylated cytosines (freqT). These data allow for direct calculation of methylation levels and coverage-based filtering for downstream analyses. File naming convention: [Genotype]_[Replicate].Original_Meth_Site.zip (e.g., HCT_DP_1.Original_Meth_Site.zip). Each archive contains three files named as [Genotype]_[Replicate]_[Context].txt (e.g., HCT_DP_1_CG.txt).



