File S1 - Lactobacillus gasseri SF1183 Affects Intestinal Epithelial Cell Survival and Growth

Supplemental figures. Figure S1, the CM of L. gasseri was size fractionated with a 3 kDa molecular mass cut-off filter and loaded on a gel filtration chromatographic column (Superdex Peptide 10/300 GL, GE Healthecare Life Sciences). The elution buffer was AMAC 0.3 M. Two main peaks were observed at 220 nm. Figure S2, chromatographic fractions from the experiment of Fig. S1 were tested by western blotting with anti-PARP-1 antibody (A). As a control, cells were also treated with the elution buffer (AMAC 0,3 M). (B) Densitometric analysis of the western blot. PARP-1 band intensity was evaluated by ImageQuant analysis on at least two different expositions to assure the linearity of each acquisition. Values are expressed as ratio with the corresponding actin values and normalised to the reference point (PARP-1 cleavage in medium). Percentage of increase (+) or decrease (–) with respect to the intensity of the reference point are indicated. Figure S3, enlargment of part of Fig. 4 showing the output of the FACS analysis. Figure S4, enlargment of part of Fig. 5 showing the output of the FACS analysis. (PDF)