Dysregulation of different classes of tRNA fragments in chronic lymphocytic leukemia

We analyzed small RNA sequencing data from CD5+/CD19+ B cells of a cohort of indolent and aggressive CLL patients compared with CD19+ B-cells of healthy donors. We identified tsRNA signatures in indolent and aggressive CLL vs. normal B-cells; we also found a drastic dysregulation of the expression of mature tRFs in CLL. Overall design: A total of 20 whole blood samples provided by the CLL Research Consortium and grouped into the following two categories: 10 indolent CLL and 10 aggressive CLL. A third category of 8 CD19+ B-cell control samples (courtesy of J. Byrd MD, IRB approved protocol) was also considered in the study.