Gene expression in non-perfused liver samples from endotoxemic Sus scrofa in context of deoxynivalenol contaminated diets

It has been proposed that chronic exposure to deoxynivalenol modulates hepatic gene expression and that sensivity to xenobiotics like deoxynivalenol is modulated by inflammation. We used microarrays to evaluate whether chronic deoxynivalenol exposure influences the hepatic gene expression of Sus scrofa in vivo and whether interaction between chronic deoxynivalenol exposure and systemic inflammation occur. Sus scrofa liver samples were collected at slaugthering 195 minutes after inducing endotoxemia via pre-hepatic or post-hepatic LPS infusion. Animals were subjected to chronic deoxynivalenol exposure via diet (4.59 mg deoxynivalenol/kg diet) or to a control diet (0.12 mg deoxynivalenol/kg diet) for 29 days before LPS infusions. RNA of nin-perfused liver samples was isolated and subjected to Affymetrix microarrays