Glutamine synthetase gene glnA plays an important role in curdlan biosynthesis of Agrobacterium sp. CGMCC 11546

Purpose: The goal of this study is compare the effect of glnA gene in curdlan synthesis in Agrobacterium sp. CGMCC 11546. methods: The transcriptional and metabolomics analysis the function of glnA in Agrobacterium sp. CGMCC 11546. Results: The transcriptional and metabolomics showed that the decrease of curdlan production in the ?glnA mutants may be caused by the insufficient supply of energy ATP conclusion: glnA play an important role in curdlan synthesis in Agrobacterium sp. CGMCC 11546 Overall design: The total RNA of each sample from the wild type strain and the gene deletion mutants after 24 hours fermentation, samples from glnA deletion mutant named glnA-1, glnA-2 and glnA-3; three biological repeats. samples from the wild type strain named WT-1, WT-2 and WT-3; three biological repeats