vGLUT1 excitatory presynaptic terminal transcriptome

In order to characterize transcripts and translational machinery in excitatory presynaptic terminals, we used our recently developed platform that couples fluorescence-sorting with biochemical fractionation to sort and purify fluorescently labeled synaptosomes (fluorescence-activated synaptosome sorting; FASS). The “pre-sorted” and sorted synaptosomes comprise resealed presynaptic synaptic compartments, sometimes associated with an “open” postsynaptic membrane ; the sorted synaptosomes also lacked dendritic and ER elements. Starting with the forebrain of adult vGLUT1venus knock-in mice, in which all vGLUT1+ synapses were fluorescently labeled, we prepared and sorted vGLUT1+ synaptosomes for RNA sequencing.