Biphasic Cell Cycle Defect Causes Impaired Neurogenesis in Down Syndrome [Pluripotency, RNA-Seq]

Using integrative approach involving human and mouse iPSCs, we have unravelded the cellular and molecular mechanisms of reduced neurogenesis in Down syndome 2N and Ts65Dn mouse embryonic fibroblasts (MEFs) were used to generate 2N and Ts65Dn induced pluripotent stem cells (iPSCs) respectively. In addition to these cells, mouse embryonic stem cells ESD3 were used for total RNA preparation. A toal of 15 samples having 3 replicates of each that is 2N MEFs, Ts65DN MEFs, 2N iPSCs, Ts65Dn iPSCs and ESD3 were used for mRNA seq library preperation. libraries were subjected to paired end high thoughput sequencing.