Sirex noctilio Transcriptome or Gene expression

Sirex noctilio, an exotic woodwasp recently introduced into the U.S., places economically-important pine species at risk of colonization by a fungal pathogen and subsequent death. The goal of this project is to isolate the a wilt-inducing factor (WIF) from Sirex noctilio venom and to elucidate the mechanism(s) by which WIF and other constituents suppress defense responses in pine to facilitate fungal colonization and subsequent tree mortality. The WIF is a diffusible small peptide isolated from S. noctilio venom which causes physiological changes that weaken pine defenses. This transcriptomic approach was used to identify the putative protein constituents of woodwasp venom. Specifically, RNA-Seq analysis of S. noctilio venom glands was used to generate a database of venom putative protein constituents for comparison with venom transcriptomes and genomic sequences recently produced for aculeate wasp species. The resulting transcript assemblies represent the first Symphyta sequence dataset available for comparative genomic studies within the Hymenoptera. (Symphyta, a major hymenopteran suborder, contains many major tree and agricultural plant pests, including sawflies.)

Sirex noctilio，一种近期被引入美国的异国木蜂，使得经济价值较高的松树物种面临真菌病原体入侵和随后死亡的威胁。本项目的目标是从Sirex noctilio的毒液中分离出导致萎蔫的因子（WIF），并阐明WIF及其他成分抑制松树防御反应的机制，从而促进真菌的定殖和树木的死亡。WIF是一种可扩散的小肽，从S. noctilio的毒液中分离出来，能够引起生理变化，削弱松树的防御能力。本研究采用了转录组学方法来鉴定木蜂毒液中的潜在蛋白质组分。具体而言，通过对S. noctilio毒液腺体的RNA-Seq分析，构建了一个毒液潜在蛋白质组分的数据库，用于与近期为螫刺蜂类物种产生的毒液转录组和基因组序列进行比较。所得的转录组装代表了Hymenoptera类群中Symphyta序列数据集的首次构建，而Symphyta是Hymenoptera的主要亚目，其中包含了许多重要的树木和农业植物害虫，如锯蜂。