Melt-MAMA primers targeting canonical SNPs for 8 new phylogenetic branches discovered in this studya.

aMelt-MAMA, melt-mismatch amplification mutation assay; SNP, single nucleotide polymorphism; con, concentration.bAmes Ancestor reference genome (NC_006570).cSNP states are presented according to the top strand in the Ames ancestor AE017334 D: Derived SNP state; A: Ancestral SNP state.dMelt-mismatch amplification mutation assay (MAMA), A: Ancestral; D: Derived; C: Common. Primer tails and antepenultimate or penultimate mismatch bases are in lower case.eFinal concentratinon of each primer in Melt-MAMA genotyping assays.