Genome-wide DNA-binding analysis for E(spl)m8 in intestinal stem cells by DamID. Drosophila melanogaster

Purpose: Genome-wide DNA-binding analysis for E(spl)m8 in intestinal stem cells in Drosophila midgut by DNA adenine methyltransferase identification(DamID) Methods: DNA adenine methyltransferase identification (DamID) on E(spl)m8 driven by Dl-Gal4 Results: Overall design: Dam(Ctrl) or E(spl)m8-Dam transgenes were expressed in intestinal stem cells by Dl-Gal4.The guts within 2 days was used for DamID-seq, followed by deep sequencing analysis.