Single-cell RNA-seq profiling of Pseudomyxoma Peritonei

Through single-cell transcriptomic profiling of five samples with Pseudomyxoma Peritonei (PMP) , we identified ten distinct cell type-specific clusters, including an epithelial cell community with significantly increased expression of canonical epithelial molecules, and a mesenchymal cell community with notably higher expression of established mesenchymal molecules, supporting the presence of both epithelial and mesenchymal molecular characteristics in PMP disease. Single-cell transcriptomic profiling was performed in a patient group with five PMPs to verify cell type-specific gene expression patterns.