The rice anther transcriptomes responded to low temperature [RNA-seq]

The booting stage of rice shows the most sensitivity to cold stress, and low-temperature stress causes irreversible pollen sterility. We performed transcriptome analysis using RNA-seq to investigate the response of rice anthers to low-temperature stress. In this RNA-seq analysis, to validate the results of transcriptome analysis of anthers from 13 rice lines using microarrays, four cultivars were selected for analysis from the 13 tested for microarrays. The four rice lines exposed to the low temperature at 12℃ for four days, were used for the RNA-seq. RNA was extracted from the anthers after the cold-treated and non-treated anthers with two replicates.