Supporting Information S1 - Copper Oxide Nanoparticles Induced Mitochondria Mediated Apoptosis in Human Hepatocarcinoma Cells

Morphology of HepG2 cells exposed to different concentrations of CuO NPs for 24 h. (A) control, (B) 2 µg/ml, (C) 5 µg/ml, (D) 10 µg/ml, (E) 25 µg/ml and (F) 50 µg/ml. Figure S2, Effect of dissolved Cu2+ on the viability and morphology of HepG2 cells. (A) MTT assay and (B) NRU assay. Data represented are mean ± SD of three identical experiments made in three replicate. *Statistically significant difference as compared to the controls (p<0.05 for each). (C) Morphology of HepG2 cells exposed to Cu2+ at the concentration of 5.0 µg/mL for 24 h.
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