GFP-LC3 puncta synthesis and degradation in treatment with full media, aa-starvation, and glucose-starvation.

Average GFP-LC3 puncta per cell for cells treated with FM (tab1), amino acid starvation (tab 2), or glucose starvation (tab 3) with or without BafA1. Data captured in 2-hour intervals (1st, 2nd, 3rd datasets indicated). Synthesis and degradation quantified as described in Methods (and SD and SEM calculated using propagation of error rules). The cumulative (stitched) data from time 0 combines the synthesis and degradation values from all 3 datasets into one continuous plot. (XLSX)