Wildtype and PALS1 knockout MDCKII cells grown under non-confluent and in 3D cyst cultures

To investigate how gradual differences in the tight junction formation could be linked to an altered gene expression, wildtype and PALS1 knockout cell lines were grown under non-confluent and in 3D cyst cultures. After preparation of the mRNA samples, mRNASeq was performed. Normalized gene expression data was then was analyzed for differentially expressed genes, and GO-Term enrichment studies were used to elucidate involved pathways. Wild-type (WT) MDCK cells and MDCKII cells with a targeted knock-out in the PALS1 gene (PALS1 KO) were grown under different growth conditions (non-confluent and in 3D cysts).