Dynamic panoramic presentation of skin function after fractional CO2 laser treatment

Fractional CO2 laser, as a typical ablative laser, has been used to assist in the treatment of many skin diseases, such as photoaging, atrophic scar, hypertrophic scar, superficial pigmentation, vitiligo, and so on. However, the dynamic changes in skin function after fractional CO2 laser treatment are still unclear. This study explored the changes in local skin function and possible regulatory mechanisms after fractional CO2 laser treatment for 1, 3, 5 and 7 days through transcriptome high-throughput sequencing. The results showed that fractional CO2 laser tended to transform the “lesions” into “normal skin”, regulate the skin barrier, coordinate the rearrangement of collagen, enhance the local microvascular circulation, activate the immune system to secrete a large number of cytokines, and act as an auxiliary tool to assist drug transport. According to the basic principle of destruction before reconstruction, fractional CO2 laser plays a key role of balancer in skin reconstruction. In conclusion, the gene expression profiles of fractional CO2 laser-treated skin undergo dynamic changes over time. Skin barrier, collagen arrangement, drug metabolism, immune system and skin defense system all present obvious characteristics of timing change after fractional CO2 laser treatment. Overall design: A total of 12 guinea pigs were used in the experiment, and were divided into 4 time points for testing and sampling (3 guinea pigs treated each time). After adapting to the environment for four days, the back skin of guinea pigs was depilated one day before a single fractional CO2 laser treatment.The right side was irradiated with a single fractional CO2 laser (Kinglaser, KL type, JiLin, China). The energy of the laser is 140 mJ, the size of the spot is 2 x 2 cm2, and the spacing between the beams is 0.6 mm, while the left of back skin was not irradiated. On day 1, 3, 5 and 7 after treatment, Skin tissue was collected separately.