Transcriptome analysis identifies key genes involved in anthocyanin biosynthesis in black and purple fruits (<i>Lycium ruthenicum</i> Murr. L)

<i>Lycium ruthenicum</i> is a characteristic plant resource in Northwest China. The fruit with high anthocyanin content is the main reason for its high nutritional value and economic value, while the regulation of key genes regulating anthocyanin biosynthesis in black fruit is not clear. In this study, high-throughput sequencing technology was used to compare the expression differences of all genes in black and purple fruits. The purple samples obtained 45.13 and 51.49 M Raw reads, while the black fruit samples measured 53.85 and 47.38 M Raw Reads. After splicing and assembly, 162,842 transcripts were obtained after removing redundant and duplicated results. Homology comparison of all predicted proteins found that 36,978 (22.43%) genes in <i>Lycium ruthenicum</i> were homologous to <i>Solanum tuberosum</i>, followed by 24,459 (14.84%) genes were homologous to <i>Capsicum annuum</i>. Overall, 3685 genes were up-regulated in purple fruit, 2837 genes were down-regulated in purple fruit, and the remaining 156320 genes were not differentially expressed in black and purple fruit. Through homology comparison of anthocyanin biosynthesis and metabolism structure genes in <i>Lycium ruthenicum</i>, 63 homologous genes, 4 MYB transcription factors and 5 bHLH transcription factors were screened. <i>LrAN2p</i> (909 bp) in purple fruit was 136 bp more upstream of ‘ATG’ than <i>LrAN2</i> (774 bp) in black fruit. Three highly expressed copies of bHLH transcription factors <i>AN1b</i> with different lengths were screened in black fruit, but they were almost not expressed in purple fruit. This study possibly identified the major <i>LrAN1b</i> genes regulating the black fruit trait.

黑果枸杞（Lycium ruthenicum）是中国西北特有的植物资源。其果实富含花青素，这是其具备高营养价值与经济价值的核心缘由，但黑果果实中调控花青素生物合成的关键基因的表达调控机制仍未明晰。本研究采用高通量测序技术，对比分析黑果与紫果枸杞的全基因表达差异。紫果样本获得45.13与51.49 M条原始读段（Raw reads），黑果样本则获得53.85与47.38 M条原始读段。经序列拼接与组装，并去除冗余与重复序列后，共获得162,842条转录本。对所有预测蛋白进行同源比对后发现，黑果枸杞中有36,978个基因（占比22.43%）与马铃薯（Solanum tuberosum）同源，其次为24,459个基因（占比14.84%）与辣椒（Capsicum annuum）同源。整体而言，紫果中共有3685个基因呈上调表达，2837个基因呈下调表达，剩余156,320个基因在黑果与紫果间无显著表达差异。通过对黑果枸杞中花青素生物合成与代谢通路的结构基因进行同源比对，共筛选得到63个同源基因、4个MYB转录因子与5个bHLH转录因子。紫果中的LrAN2p（长度909 bp）相较于黑果中的LrAN2（长度774 bp），其ATG起始密码子上游序列多出136 bp。本研究在黑果中筛选得到3个长度各异且高表达的bHLH转录因子AN1b拷贝，但这些拷贝在紫果中几乎不表达。本研究或已明确调控黑果性状的关键LrAN1b基因。

搜集汇总

背景与挑战

背景概述

该数据集基于转录组分析，旨在识别黑果枸杞中调控花青素生物合成的关键基因，通过比较黑果和紫果的基因表达差异，筛选出包括LrAN2p和AN1b在内的关键基因和转录因子。数据集包含两个Excel文件，提供原始测序数据和基因表达分析结果，适用于植物生物学和遗传学研究。