Dietary metabarcoding datasets for the southern meadow jumping mouse (Zapus luteus luteus)

The southern meadow jumping mouse (Zapus luteus luteus) is Endangered in the US due to loss of riparian habitat and knowledge of diet can focus conservation efforts. Context and life stages of diet have been documented for other species of Zapus (seeds, insect larvae, and hypogeous fungi), possibly shifting toward greater diversity in seed predations prior to hibernation. Yet, challenges in field observation, thorough digestion, and lack of scalability have hindered a thorough survey of dietary taxonomies. We collected the feces of 165 Z. l. luteus from across the geographic range. Emphasizing granivorous diet, we used DNA metabarcoding to resolve its dietary taxonomies. With negligible impact of field contamination, we found a varied diet most frequently of graminoids, forbs, and lepidoptera. Detecting up to nine dietary taxa in individuals, Z. l. luteus consumed more and different combinations of plant taxa as they approached the known hibernation window. Our work emphasizes the impor...