Data and code from: Early developmental decline in HSP expression affects subsequent response to transient heat exposure

Understanding physiological responses to short-term changes in temperature is of growing interest, considering the rising frequency and severity of transient temperatures such as heat waves. During the embryonic development of egg-laying vertebrates, inducible physiological responses to transient heat are likely critical to short-term survival but may also be energetically costly or disruptive to development. Inducible heat-shock proteins (HSPs) are conserved molecular chaperones that act to safeguard cellular protein homeostasis during transient stress. However, experiments in ectotherms have shown that overexpression of HSPs can increase embryonic mortality and reduce later thermotolerance. Yet, few studies have explored natural developmental patterns of HSP expression and heat inducibility in embryos of egg-laying ectothermic vertebrates. Using the red-eared slider turtle (Trachemys scripta), we characterized the response of five HSP genes in embryonic trunks following repeated 3-d t..., , # Data and code from: Early developmental decline in HSP expression affects subsequent response to transient heat exposure

## Descriptions of the Data and file structure

There are three separate Excel data files corresponding to each of the three studies/experiments described in the manuscript. These are referred to as 'StudyIData', 'StudyIIData', and 'StudyIIIData', corresponding respectively with study I, study II, and study III of the manuscript. Each Excel file contains gene expression values of 5 HSP genes (normalized to the housekeeping gene) as obtained from biological (embryonic trunks) samples in each study.

**Excel Document \"StudyIData.xlsx\" Headers:**

**Sheet 1 'StudyI_MainSheet'**

* EggID: identifies individual T. scripta eggs (i.e., embryos) used in the study. Note: these are distinct from eggs used in the other studies.
* Treatment: the treatment applied to that specific egg. 2 levels: RecurringHeat or CoolControl; individuals underwent either three repeated 3-day hea...,