Characterization of CD146+ pericytes isolated from wild-type and mdx mice: proliferation, gene expression profile, differentiation potential, and secretome data

[ENG:]This dataset supports a project investigating differences in the proliferative capacity, gene expression profile, protein synthesis, differentiation potential, and secretome composition of CD146-positive (CD146+) pericytes isolated from skeletal muscles of wild-type (WT) and mdx mice, the latter serving as a model for Duchenne muscular dystrophy (DMD).CD146+ pericytes were isolated from hindlimb skeletal muscles of WT and mdx mice and enriched using magnetic-activated cell sorting (MACS) based on CD146 surface expression. In vitro proliferation was assessed using the CFSE assay. To analyze the gene expression profile, total RNA was extracted from cultured CD146⁺ cells, reverse-transcribed to cDNA, and analyzed by quantitative PCR (qPCR). Protein-level analyses were performed using western blotting to quantify selected markers.Differentiation potential was evaluated under defined adipogenic, fibrogenic, and myogenic culture conditions. The secretome was analyzed through its functional impact on other cell types using an in vitro angiogenesis assay. Additionally, conditioned medium was collected from CD146⁺ cells to assess the expression of secreted factors.This dataset includes:.xlsx and .txt filesSpecifically:"2way_ANOVA_of_CFSE_24h"; - text file (.txt) showing statistical analysis (two-way ANOVA) of the percentage of proliferating cells"2way_ANOVA_of_CFSE_48h" - text file (.txt) showing statistical analysis (two-way ANOVA) of the percentage of proliferating cells"angiogenesis_raw_data" - Excel file containing raw counts of angiogenesis assay"cFos_tubulin_WB_raw_data" - Excel file containing raw measurements of tubulin band intensity for cFos analysis"cFos_WB_raw_data" - Excel file containing raw measurements of cFos band intensity"cJun_tubulin_WB_raw_data" - Excel file containing raw measurements of tubulin band intensity for cJun analysis"cJun_WB_raw_data" - Excel file containing raw measurements of cJun band intensity"NFkB_tubulin_WB_raw_data" - Excel file containing raw measurements of tubulin band intensity for NFkB analysis"NFkB_WB_raw_data" - Excel file containing raw measurements of NFkB band intensity"phos_cFos_tubulin_WB_raw_data" - Excel file containing raw measurements of tubulin band intensity for phos-cFos analysis"phos_cFos_WB_raw_data" - Excel file containing raw measurements of phos-cFos band intensity"phos_cJun_tubulin_WB_raw_data" - Excel file containing raw measurements of tubulin band intensity for phos-cJun analysis"phos_cJun_WB_raw_data" - Excel file containing raw measurements of phos-cJun band intensity"phos_NFkB_tubulin_WB_raw_data" - Excel file containing raw measurements of tubulin band intensity for phos-NFkB analysis"phos_NFkB_WB_raw_data" - Excel file containing raw measurements of phos-NFkB band intensity"fibrogenesis_mdx" - Excel file with raw measurements of collagen deposition area by mdx cells"fibrogenesis_WT" - Excel file with raw measurements of collagen deposition area by WT cells"Unpaired_t_test_of_fibrogenesis" - text file (.txt) showing statistical analysis of the collagen deposition area"fusion_index_mdx" - Excel file with raw nuclei count of differentiating mdx cells"fusion_index_WT" - Excel file with raw nuclei count of differentiating WT cells"Unpaired_t_test_of_fusion_index" - text file (.txt) showing statistical analysis of the fusion index"luminex_raw_data" - Excel file with raw data from secretome composition (luminex) analysis"markers_mdx_CD146_Abs_Quant" - text file containing raw Cq data for gene expression in mdx cells"markers_WT_Abs_Quant" - text file containing raw Cq data for gene expression in WT cells"markers_MDX" - Excel file with the percentage of mdx cells expressing surface markers analyzed by flow cytometry"markers_WT" - Excel file with the percentage of WT cells expressing surface markers analyzed by flow cytometry"Ordinary_one_way_ANOVA_of_junctions" - text file (.txt) showing statistical analysis (one-way ANOVA) of the number of junctions"Ordinary_one_way_ANOVA_of_meshes" - text file (.txt) showing statistical analysis (one-way ANOVA) of the number of meshes"Ordinary_one_way_ANOVA_of_nods" - text file (.txt) showing statistical analysis (one-way ANOVA) of the number of nods"Ordinary_one_way_ANOVA_of_segments" - text file (.txt) showing statistical analysis (one-way ANOVA) of the number of segments"Ordinary_one_way_ANOVA_of_total_lenght" - text file (.txt) showing statistical analysis (one-way ANOVA) of the total lenght of the mesh"Ordinary_one_way_ANOVA_of_total_segment_lenght" - text file (.txt) showing statistical analysis (one-way ANOVA) of the lenght of the segments"perilipin_mdx" - Excel file with raw counts of perilipin expressing mdx cells"perilipin_WT" - Excel file with raw counts of perilipin expressing WT cells"Unpaired_t_test_of_perlipin" - text file (.txt) showing statistical analysis of the perilipin positive cells"proliferation_24h_raw_data" - text file with the raw counts of the cells treated with CFSE, 24h of treatment"proliferation_48h_raw_data" - text file with the raw counts of the cells treated with CFSE, 48h of treatment"Unpaired_t_test of Cspg4" - text file with the statistical analysis of the differences in the Cspg4 expression"Unpaired_t_test_of_Alp" - text file with the statistical analysis of the differences in the Alp expression"Unpaired_t_test_of_Mcam" - text file with the statistical analysis of the differences in the Mcam expression"Unpaired_t_test_of_Myod" - text file with the statistical analysis of the differences in the Myod expression"Unpaired_t_test_of_Nes" - text file with the statistical analysis of the differences in the Nes expression"Unpaired_t_test_of_Pax7" - text file with the statistical analysis of the differences in the Nes expression"Unpaired_t_test_of_Pdgfra" - text file with the statistical analysis of the differences in the Pdgfra expression"Unpaired_t_test_of_Pdgfrb" - text file with the statistical analysis of the differences in the Pdgfrb expression"Unpaired_t_test_of_Peg3" - text file with the statistical analysis of the differences in the Peg3 expression"Unpaired_t_test_of_ANGPT_2" - text file with the statistical analysis of the differences in the Angpt2 secretion"Unpaired_t_test_of_ANGPT1" - text file with the statistical analysis of the differences in the Angpt1 secretion"Unpaired_t_test_of_SDF1" - text file with the statistical analysis of the differences in the Sdf1 secretion"Unpaired_t_test_of_c_Fos" - text file with the statistical analysis of the differences in the cFos protein level"Unpaired_t_test_of_c_Jun" - text file with the statistical analysis of the differences in the cJun protein level"Unpaired_t_test_of_NFkB" - text file with the statistical analysis of the differences in the NFkB protein level"Unpaired_t_test_of_phos_c_Fos" -text file with the statistical analysis of the differences in the phosphorylated isoform of cFos protein level"Unpaired_t_test_of_phos_c_Jun" - text file with the statistical analysis of the differences in the phosphorylated isoform of cJun protein level"Unpaired_t_test_of_phos_NFkB" - text file with the statistical analysis of the differences in the phosphorylated isoform of NFkB protein level"Unpaired_t_test_of_Pdgfra_fcs" - text file with the statistical analysis of the differences in the percentage of Pgdfra positive cells"Unpaired_t_test_of_Pdgfrb_fcs" - text file with the statistical analysis of the differences in the percentage of Pgdfrb positive cells"Unpaired_t_test_of_CD146_fcs" - text file with the statistical analysis of the differences in the percentage of CD146 positive cells