Total RNA from NF cells treated with or without P4 for 96h was extracted according to the manufacturer's protocol. RNA was subjected to RNA-Seq analysis.
Sequence data of HeLa cells were generated based on ribominus RNA sequencing with or without RNase R treatment (RNaseR+/-), respectively. These data sets were used for prediction of circRNAs.
ACH-2 cells were analyzed in presence and abscenece of fentanyl. Cells were treated with 10ug of fentanyl for 24 hours. ACH-2 cells without fentanyl treatment is considered as control. Both control an