Chip-seq analysis of meiotic Cohesin complexes (mei-Cohesins) subunits, CTCF, and BORIS/CTCFL in Macaca fascicularis testis

The study involved chromatin IP analyzed by high-throughput sequencing analysis using purifird germ cells from M. fascucularis testis M. fascicularis is a monkey with non-seasonal male germ cells production, and the most popular primate for preclinical animal studies. The goal of the study was to investigate the epigenome of M. fscicularis testis with respect to mei-Cohesin complexes, CTCF and BORIS Overall design: The cynomolgus monkeys testis material was collected from six 7-9 years old male monkeys. The testes were placed on ice and germ cells were prepared by passing through two mesh sizes.The cell fraction was fixed according to ChIP-seq protocol and kept frozen