Stromal Cells from Endometriosis Patients Reflect Lesion Type-Specific Heterogeneity

Endometriosis shows significant lesion heterogeneity, but pathophysiological mechanisms leading to differences in clinical presentation are poorly understood. Here we utilized primary stromal cells from different types of endometriotic lesions to better understand the molecular mechanisms that lead to endometriosis heterogeneity. For this project, endometrial stromal cells (ESCs) were obtained from eutopic endometrium (EU), endometrioma (OMA), superficial (SF) and deep (DE) endometriosis lesions and analyzed in vitro for their behavior regarding proliferation, migration, and contractility. RNA sequencing was performed to explore the molecular mechanism underlying the observed changes. Overall design: RNASeq of endometrial stromal cells from eutopic endometrium (C), deep endometriosis (DE), endometrioma (OMA), and superficial endometriosis (SF).