Independent and differential effects of arbuscular mycorrhizal fungal composition and plant pathogens on plant traits and nitrogen uptake

Arbuscular mycorrhizal fungi (AMF), as microbial mutualists, interact with various microbial taxa, including pathogens, and significantly shape the ecology and evolution of their host species. However, how AMF and pathogens jointly or independently affect plant traits and nitrogen uptake remains unclear. Here we conducted a factorial experiment with three AMF treatments (AMF-free-control, Funneliformis mosseae, and a mixture of AMF species of F. mosseae and Claroideoglomus etunicatum), four plant-pathogen pairs, each under two pathogen treatments (one pathogen and a pathogen-free control). After 65 days of growth, we measured AMF colonization, pathogen infection, plant functional traits, and ammonium and nitrate uptake. Our findings reveal that AMF and pathogens independently influence plant traits and nitrogen uptake, with no observed interactions between them. Specifically, colonization by F. mosseae or a mixed AMF species reduced nitrate uptake and plant height, without affecting roo..., , , # Independent and differential effects of arbuscular mycorrhizal fungal composition and plant pathogens on plant traits and nitrogen uptake

This file includes all the data and code of the paper about \"Tripartite interactions among plants, pathogens, and AMF\" for readers. The descriptions of the column names of data (excel name: data_Pathogen and N uptake) we used for statistical analysis.

SP: species name
QM: *Bromus japonicus*
PJ: *Elymus nutans*
MX: *Medicago sativa*
DD: Glycine max
AMF_T: AMF treatments (control, *Funneliformis mosseae*, and mixed strain of *F. mosseae* and *Claroideoglomus etunicatum*)
Fm: *Funneliformis mosseae* colonization treatment
M: mixed strain of *F. mosseae* and *Claroideoglomus etunicatum* colonization treatment
C: AMF- or pathogen-free control
Dis_T: pathogen treatments (one pathogen and a pathogen-free control)
Pa: pathogen infection treatment
N_form: N form of plant uptake
AM: NH4+
NT: NO3-
T: Treatment
CK: AMF- and pathogen-free control; Pa: AMF-free...